2015 Fiscal Year Final Research Report
Visualization and manipulation of stress-activated MAPK signaling for understanding of stress-dependent cell fate determination system.
| Project/Area Number |
25440043
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Toho University (2015)
The University of Tokyo (2013-2014) |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | イメージング / MAPK / ストレス応答 |
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| Outline of Final Research Achievements |
Quantitative analysis of stress-activated MAPK signaling in cultured cells were done using FRET based sensors. I found that inflammatory cytokines induces oscillatory p38 activation in cytoplasm, and demonstrated that such p38 activation occurs heterogeneously in cells. Gene expression analysis further revealed that oscillatory p38 activities efficiently induces expression of pro-inflammatory cytokine gene expression than continuously elevated p38 activity does. When p38 activity was introduced in cells by inducible rapamycin-dependent dimerization of FRB domain and FKBP-p38 fusion protein, we found that there is some relationship beteween p38 activity and bleb-like plasma-membrane structure even in the absence of upstream signal activation. Such membrane structure was also seen when cells were stimulated by UV stresses or by applying pro-inflammatory cytokines. Thus, this study revealed unexpected p38 signal dynamics as well as its role in inflammatory signaling.
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| Free Research Field |
分子生物学、生理学、薬理学
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