Analysis on signla transduction with membrane receptor-/ effector protein-reconsituted artificial cell systems

2015 Fiscal Year Final Research Report

• Project/Area Number: 25440045
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Mie University
• Principal Investigator: Tsumoto Kanta 三重大学, 工学(系)研究科(研究院), 准教授 (80362359)
• Co-Investigator(Renkei-kenkyūsha): KIDOAKI Satoru 九州大学, 先導物質化学研究所, 教授 (10336018)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 人工細胞モデル / 細胞情報伝達 / リポソーム / 膜タンパク質 / バキュロウイルス / プロテオリポソーム / GUV / アデニル酸シクラーゼ

Outline of Final Research Achievements

We tried developing a protocol to reconstitute GPCRs and following other elements of cell signal transduction pathways on artificial lipid membrane vesicles such as giant liposomes for realizing microscopic observation on function of the pathway with individual GUVs. We used our method to prepare giant proteo-liposomes through membrane fusion between GUVs and envelopes of recombinant baculovirus budded virus particles, and demonstrated GUVs containing recombinant receptors, effectors, or some proteins following these in actual cells, by microscopic observation. It is now difficult to construct the full pathway; however, we have obtained results about the availability of GUVs formed with a droplet-transfer method and characterization of budded viruses prepared using some culture procedures, which give useful information to improve our study on reconstitution of membrane protein systems on giant proteoliposomes.

Free Research Field

分子生物工学