Molecular mechanism for bipolar filament formation of type 2 myosin

2015 Fiscal Year Final Research Report

• Project/Area Number: 25440061
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biophysics
• Research Institution: Hokkaido University
• Principal Investigator: Takahashi Masayuki 北海道大学, 理学(系)研究科(研究院), 准教授 (50241295)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: ミオシン / フィラメント / 自己会合 / 細胞骨格 / 細胞運動

Outline of Final Research Achievements

Nonmuscle myosin II molecules are able to function by forming a bipolar filament in a cell under spatiotemporal control. We focused on the roles of some charge clusters in the rod region of myosin II molecule and tried to clarify the molecular mechanism of the bipolar filament formation. We revealed that two positive charge clusters close to the C-terminal end play an important role on the electrostatic interaction in assembly processes. In addition, we were able to capture the dynamic behaviors of myosin IIA and myosin IIB isoforms in a migrating cell simultaneously.

Free Research Field

生物化学