2015 Fiscal Year Final Research Report
Development and application of the experimental platform for the single-molecule analysis of the lipid-effects on the ion channel function
| Project/Area Number |
25440067
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | University of Fukui |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 生体膜 / 1分子計測 / チャネル / 脂質 / 電気生理 |
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| Outline of Final Research Achievements |
Based on the principle of formation of the contact bilayer between water-in-oil droplets, we developed the platform for the artificial membrane experiments to analyze quantitatively the "lipid-effects" on the function of the membrane proteins. We prepared a variety of lipid bilayer membranes having different lipid composition including asymmetric membranes on the platform. We reconstituted the KcsA potassium channel into these membranes and succeeded in detecting the lipid-dependency in the activity of the channel at single-molecule level.
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| Free Research Field |
イオンチャネルの分子生理学
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