2015 Fiscal Year Final Research Report
Elucidation of the structure and function of a photoactivated adenylyl cyclase from a sulfer bacterium
| Project/Area Number |
25440072
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Biophysics
|
|---|
| Research Institution | Toho University |
|---|
Principal Investigator |
ISEKI Mineo 東邦大学, 薬学部, 准教授 (60414009)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Keywords | 光センサー / フラビン / アデニル酸シクラーゼ / cAMP |
|---|
| Outline of Final Research Achievements |
This research is aimed at elucidating the activation mechanism of photoactivated adenylyl cyclase (PAC). We found a new homolog of PAC in the genome sequence of a cyanobacterium Oscillatoria acuminata and demonstrated that the homolog (OaPAC) can be easily expressed and purified from E. coli as soluble form. Using the purified protein, we successfully determined the photochemical and biochemical properties of the protein, and solved its crystal structure under collaboration with structure biologists.
|
| Free Research Field |
光生物学
|
