• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2015 Fiscal Year Final Research Report

Elucidation of the kinase module to control the endoplasmic reticulum stress response

Research Project

  • PDF
Project/Area Number 25440078
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Cell biology
Research InstitutionUniversity of Tsukuba

Principal Investigator

Mizuno Tomoaki  筑波大学, 医学医療系, 助教 (80529032)

Project Period (FY) 2013-04-01 – 2016-03-31
Keywords小胞体ストレス / MAPキナーゼ / AMP活性化キナーゼ
Outline of Final Research Achievements

Accumulation of unfolded proteins in the lumen of the endoplasmic reticulum (ER) causes ER stress. Snf1, the Saccharomyces cerevisiae ortholog of AMP-activated protein kinase (AMPK), plays a crucial role in the response to various environmental stresses. However, the role of Snf1 in ER stress response remains poorly understood. In this study, we characterize Snf1 as a negative regulator of Hog1 MAPK in ER stress response. The snf1 mutants showed the ER stress resistant phenotype. Activated Hog1 levels were increased by snf1 mutation. Ssk1, a specific activator of MAPKKK functioning upstream of Hog1, was induced by ER stress and its induction was inhibited in a manner dependent on Snf1 activity. Furthermore, we show that the SSK1 promoter is important for Snf1-modulated regulation of Ssk1 expression. Our data suggest that Snf1 downregulates ER stress response signal mediated by Hog1 through negatively regulating expression of its specific activator Ssk1 at the transcriptional level.

Free Research Field

ストレス応答シグナル伝達経路

URL: 

Published: 2017-05-10  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi