Visualization of cell fates using degron based fluorescence probes.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25440095
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cell biology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: SAKAUE ASAKO 国立研究開発法人理化学研究所, 脳科学総合研究センター, 研究員 (90462689)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 多細胞社会 / 生命現象可視化 / デグロン / 蛍光タンパク / Fucci

Outline of Final Research Achievements

To understand the spatio-temporal dynamics of cellular functions in complex multi-cellular systems, we have developed a variety of fluorescent indicators for live cell imaging. The indicators include a Fucci probe that resolves a cell cycle into 7 fractions (CC (Cell-Cycle) Rainbow), oxidative stress probes, and carbohydrate metabolism probes. Practical use of these probes gave us opportunities to realize “cell heterogeneity” and appreciate “cell personality” in many cell culture systems. Our forthcoming studies will focus on generation of flox mouse lines of the probes that allow us to better understand the spatio-temporal regulations of cellular functions in a more physiological context.

Free Research Field

生物学