Functional Analysis of grimp protein that expresses in regeneration stem cells in Enchytraeus japonensis

2016 Fiscal Year Final Research Report

• Project/Area Number: 25440116
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Nihon University
• Principal Investigator: YOSHIDA-NORO Chikako 日本大学, 生産工学部, 教授 (80311356)
• Co-Investigator(Renkei-kenkyūsha): TOCHINAI Shin 北海道大学, 理学研究科, 教授 (20111148) ; MIKAMI Toshikazu 日本大学, 歯学部, 助教 (80434075)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 再生幹細胞 / 幹細胞増殖 / タンパク質機能解析 / ヤマトヒメミミズ / 無性生殖 / 細胞分化 / 細胞接着 / 免疫組織染色

Outline of Final Research Achievements

grimp gene plays an important role in early stage of regeneration in Enchytraeus japonensis. grimp mRNA is detected transiently from 6 to 12 h post amputation only in mesodermal stem cells called neoblasts and mesodermal lineage cells. In order to clarify the function and cellular localization of grimp protein, we took approaches for the production, purification and characterization of recombinant protein, and made polyclonal antibodies. By immunohistochemistry, we found that grimp protein is expressed in the mesodermal cells in regeneration bud from 6-24 h post amputation. The target protein of the antibodies was detected by the western blotting at around predicted size.

Free Research Field

発生生物学　分子細胞生物学