2015 Fiscal Year Final Research Report
glycosyltransferases for plant specialized compounds based on the 3D structures
| Project/Area Number |
25440148
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 配糖化酵素 / フラボノイド / シロイヌナズナ / 結晶構造 |
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| Outline of Final Research Achievements |
Glycosylation plays an important role in the huge chemical diversity of plant metabolites. However, the knowledge about high-order glycosylation is limited. Four crystal structures of a flavonoid high-order glycosyltransferase UGT79B1 were dissolved. Genes encoding novel flavonoid high-order glycosyltransferases from Arabidopsis and soybean were also identified. Based on these data, amino acid residues which play crucial roles in the binding of substrates and substrate inhibition were found and biochemical analyses using mutated UGT79B1 proteins were conducted.
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| Free Research Field |
植物分子生理学
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