2015 Fiscal Year Final Research Report
Regulatory mechanism of starfish reproduction by relaxin-like gonad-simulating hormone (GSS)
| Project/Area Number |
25440151
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Morphology/Structure
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| Research Institution | Tokyo Gakugei University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 生殖腺刺激ホルモン / リラキシン / ペプチドホルモン / オーソログ / シグナル情報伝達機構 / ヒトデ |
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| Outline of Final Research Achievements |
The potential of ovarian follicle cells to response to GSS was brought by de novo synthesis of Gαs-proteins. On the basis of homology search using conservative nucleotide sequences of relaxin receptor in vertebrates, cDNA for a candidate of GSS receptor was obtained from ovarian follicle cells of the starfish Asterina pectinifera. This study also showed that 1-methyladenine was produced from S-adenosyl-[methyl-13C]-methionine, when follicle cells had been preincubated in [methyl-13C]-methionine. Furthermore, chemical structures of GSS were identified in ten species of starfish. Three kinds of RGP orthologs were found in the class Asteroidea. Based on their cysteine motifs, starfish GSS molecules are classified as member of the insulin/insulin-like growth factor (IGF)/relaxin superfamily and, more precisely, they belong to a relaxin-like peptide family. Therefore, gonadotropin in starfish is designated as relaxin-like gonad-stimulating peptide (RGP).
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| Free Research Field |
生物学
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