2016 Fiscal Year Final Research Report
Origin of the adult epithelial stem cell in the Xenopus laevis intestine and the role of Notch signaling in the stem cell regulation
| Project/Area Number |
25440160
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Morphology/Structure
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| Research Institution | Nippon Medical School |
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Principal Investigator |
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| Research Collaborator |
BUCHHOLZ Daniel R. シンシナティ大学, 生物科学科, 准教授
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Keywords | 上皮幹細胞 / 消化管 / 甲状腺ホルモン / 両生類 / 変態 / Notchシグナル経路 |
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| Outline of Final Research Achievements |
In the small intestine of Xenopus laevis during metamorphosis, which is caused by thyroid hormone (TH), most of the larval epithelial cells are removed by apoptosis whereas some cells dedifferentiate into the adult epithelial stem cells. They actively proliferate and differentiate to form the adult epithelium.
We found that during this intestinal remodeling, Notch signaling pathway is activated by TH and that inhibition of this pathway inhibits TH-induced up-regulation of Notch response gene and, more importantly, LGR5, an adult intestinal stem cell marker gene. In addition, inhibition of Notch suppressed differentiation of the stem cells into the absorptive epithelial cells, but facilitated their differentiation into the secretory epithelial cells. Our results suggest that Notch signaling plays important roles in the intestinal remodeling.
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| Free Research Field |
発生生物学、比較内分泌学
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