Study on the Bio-inspired electric nose and odor-coding theory

2015 Fiscal Year Final Research Report

• Project/Area Number: 25440164
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Animal physiology/Animal behavior
• Research Institution: Muroran Institute of Technology
• Principal Investigator: IWASA Tatsuo 室蘭工業大学, 工学(系)研究科(研究院), 教授 (00133926)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 匂い分子結合タンパク質 / 化学感覚タンパク質 / バイオ化学センサー / 生体機能指向型エレクトリックノーズ / レイリー型表面弾性波センサー / ＦＥＴセンサー

Outline of Final Research Achievements

This research project aims to make a prototype of a bio-inspired electric nose, which can detect different types of odorant molecules and distinguish them. In order to use the sensing-element of the electric nose, we plan to use odorant binding proteins of Japanese common newt, Cp-Lip1 and -Lip2 which we found and chemosensory proteins (CSPs) found in the sensillum of carpenter ant, Camponotus japonicus. The expression and purification system of the proteins from E. coli expression systems were established.The purified proteins retained their native secondary structure and binding properties. The amino acid replacement of Cp-Lip1 changed its odorant binding properties, which enable to obtain odorant-sensor elements of the different odorant-binding capacity. These sensor elements were fixed and used on a prototype of a surface acoustic wave (SAW) sensor and a field effect transistor (FET) sensor.

Free Research Field

生体分子科学