Overviewing their dynamics of peptidergic neuromodulation: From single neuron to neuronal circuit

2015 Fiscal Year Final Research Report

• Project/Area Number: 25440166
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Animal physiology/Animal behavior
• Research Institution: Nagoya University
• Principal Investigator: Abe Hideki 名古屋大学, 生命農学研究科, 准教授 (90396804)
• Co-Investigator(Renkei-kenkyūsha): OKA YOSHITAKA 東京大学, 理学系研究科, 教授 (70143360)
• Research Collaborator: SHIGA MASAKATSU ; GOTO YURI ; UMATANI CHIE
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: ペプチド放出 / 神経修飾 / GnRH / 細胞培養 / live cell imaging / 嗅球 / 視蓋

Outline of Final Research Achievements

To understand peptide release from single peptidergic neuron and their neuromodulatory actions on their target neuronal circuit, we performed following studies using fish terminal nerve-GnRH3 peptidergic neurons as a model: (i) establishment of the co-culture system of terminal nerve GnRH3 neurons and olfactory bulbar neurons that receive neuromodulatory actions of GnRH3 peptide by dissociating goldfish olfactory bulb. (ii) live-cell imaging of secretory vesicles transfer and their exocytosis from single cultured TN-GnRH3 neuron by exogenous expression of the fluorescent exocytotic sensor proteins, (iii) recording of visual stimuli (static/moving small objects)-induced single neuronal activity in in vivo from the optic tectum of immobilized medaka, and (iv) whole-cell patch-clamp recording of the enhancement of Ca2+-induced K+ current by GnRH3 peptide that will inhibit subventricular neuronal activities of the optic tectum.

Free Research Field

神経生物学