Disruption of genes related to vanadium accumulation and the study of vanadium utilization pathway

2015 Fiscal Year Final Research Report

• Project/Area Number: 25440170
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Animal physiology/Animal behavior
• Research Institution: Hiroshima University
• Principal Investigator: Ueki Tatsuya 広島大学, 理学(系)研究科(研究院), 准教授 (10274705)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 機能破壊 / 酵素 / ホヤ / 金属イオン / 生体分子 / メタゲノム / トランスクリプトーム

Outline of Final Research Achievements

In this study, using vanadium-accumulating ascidians as a model system, we performed gene knockout experiments using artificial nucleases and examination of the vanadium export pathway by reverse pathway of vanadium accumulation pathway. In addition, in order to examine more deeply on the molecular mechanism, we also performed metagenomic and transcriptomic analyses. We found the correlation between vanadium concentration and microbiome. We also found novel vanadium-binding proteins and vanadium channels that are expected to be involved in vanadium export pathway.

Free Research Field

分子生理学