2015 Fiscal Year Final Research Report
Sex-determining mechanism in medaka-related species lacking Dmy
| Project/Area Number |
25440178
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Genetics/Chromosome dynamics
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| Research Institution | Niigata University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 発生遺伝 / 性決定 / 性染色体 / 性決定遺伝子 / 進化 / メダカ |
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| Outline of Final Research Achievements |
We investigated the sex determination system and sex chromosomes in six celebensis group species. Our sex-linkage analysis demonstrated that all species had an XX-XY sex determination system, and that the Oryzias marmoratus and O. profundicola sex chromosomes were homologous to O. latipes linkage group (LG)10, while those of the other four species, O. celebensis, O. matanensis, etc., were homologous to O. lat. LG 24. The phylogenetic relationship suggested a turnover of the sex chromosomes from O. lat. LG 24 to LG 10 within this group. Sex-linkage maps suggested that the LG 24 acquired the SD function in a common ancestor of the celebensis group, and that the LG 10 SD function appeared in a common ancestor of O. mar. and O. pro. after the divergence of O. matanensis. Fine mapping and association analysis in the former two species revealed that Sox3 on the Y chromosome is a prime candidate for the SD gene, and that the Y-specific 430-bp insertion might be involved in its SD function.
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| Free Research Field |
遺伝学
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