The function of mating pheromone and the speciation in Blepharisma

2016 Fiscal Year Final Research Report

• Project/Area Number: 25440210
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biodiversity/Systematics
• Research Institution: Nara Women's University
• Principal Investigator: Harumoto Terue 奈良女子大学, 自然科学系, 教授 (80198936)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 交配フェロモン / 種分化 / 系統樹 / アルベオラータ

Outline of Final Research Achievements

To investigate the function and the action mechanism of sex pheromone (gamone) in ciliate Blepharisma, ①Isolated gamone 1 genes from several stocks of Blepharisma and identified the sites which determine the specificity of species, ② examined the localization of gamone receptors in the cells, ③ tried to identify genes involved in biosynthesis of gamone 2. In order to clarify the phylogenetic relationship of the strains of Blepharisma, ④ isolated several genes and constructed phylogenetic trees, and ⑤ investigated the specificity of gamone 1 between species, whether the pair formation between different megakaryotype strains and between different species occrred, and whether progenies between such crosses survived, and reexamined the classification of the genus Blepharisma.

Free Research Field

細胞生物学