Identification of genes controlling the contents of sesame seed lignans

2015 Fiscal Year Final Research Report

• Project/Area Number: 25450003
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Science in genetics and breeding
• Research Institution: University of Toyama
• Principal Investigator: YAMAMOTO Masayuki 富山大学, 大学院理工学研究部(理学), 講師 (10456402)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: リグナン / ゴマ / 機能性成分

Outline of Final Research Achievements

Sesamin, sesamolin and sesaminol triglucoside (STG) are major lignans accumulated in sesame seeds. In this study, to identify the genes involving accumulation and biosynthesis of the lignans, I first investigated the inheritance patterns of the lignan contents in sesame seeds using F5 and F6 generations from the cross between a sesamolin-deficient and a high lignan strains. The results indicate that the contents of sesamin and the sum of the three lignans are quantative traits and might be controlled by a number of genes. In contrast, the content of sesamolin and STG were shown to be determined by a single gene and several genes, respectively. I also showed that sesamolin-deficiency was associated with the mutation of a CYP92B14 gene which encodes a novel cytochrome p450 enzyme with biosynthetic activity to convert sesamin to sesamolim, and, unexpectedly, sesaminol.

Free Research Field

植物育種学