2016 Fiscal Year Final Research Report
Mechanisms underlying the formation of syncitial endosperm using endspermless mutants in rice
Project/Area Number |
25450005
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Science in genetics and breeding
|
Research Institution | Nagoya University |
Principal Investigator |
HATTORI TSUKAHO 名古屋大学, 生物機能開発利用研究センター, 教授 (10164865)
|
Co-Investigator(Renkei-kenkyūsha) |
NAGATO Yasuo 東京大学, 名誉教授 (10143413)
|
Research Collaborator |
KATO Hirokazu
KUROTANI Ken-ichi
MIYATA Yasuhiro
|
Project Period (FY) |
2013-04-01 – 2017-03-31
|
Keywords | 胚乳 / 穀類 / 核 |
Outline of Final Research Achievements |
In many angiosperms, the primary endosperm nucleus produced by double fertilization undergoes division without cytokinesis resulting in the formation of syncytial nuclear endosperm. In rice nucler endosperm with several thousands of free nuclei is observed 3 days after pollination. To obtain deeper insight into the regulatory mechanism, we investigated a rice endospermless mutant, enl2, which lacks endosperm tissues in its mature seeds. We found that the mutant had defects in chromosome segregation and separation of free nuclei. In addition, we identified a candidate causal gene of enl2 by NGS. The gene encoded a protein, of which orthologs in some lower eukaryote including Chlamydomonas have been reported to function in nuclear envelope fusion and meiosis.
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Free Research Field |
植物分子生物学
|