The modification of flavonoid metabolic pathway in soybean which is controlled with duplicated transcription factor genes as the targets.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25450009
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Science in genetics and breeding
• Research Institution: Saga University
• Principal Investigator: Anai Toyoaki 佐賀大学, 農学部, 教授 (70261774)
• Co-Investigator(Kenkyū-buntansha): WATANABE Satoshi 佐賀大学, 農学部, 講師 (40425541)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: ダイズ / イソフラボン / MYB / 転写制御 / 変異体

Outline of Final Research Achievements

In this study, we tried to establish a novel metabolic engineering approach to modify the key transcriptional factor, MYB, which coordinately regulate multiple target genes involved in the flavonoid metabolic pathway for modification of isoflavone content in soybean seed.
As a result, we could develop a novel heterologus transcriptional factor evaluation system for target gene promotor via agr-infiltration system of N. Benthamiana, and achieved to identify the MYB gene that regulates a target, CHS7 gene expression.

Free Research Field

植物分子育種学