Effective gene analysis system using DNA transposon, nDart

2016 Fiscal Year Final Research Report

• Project/Area Number: 25450013
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Science in genetics and breeding
• Research Institution: National Institute for Basic Biology
• Principal Investigator: Tsugane Kazoo 基礎生物学研究所, 多様性生物学研究室, 助教 (50343744)
• Co-Investigator(Renkei-kenkyūsha): Tsugane Mika (Hayashi Mika) 基礎生物学研究所, 多様性生物学研究室, 特別協力研究員 (90625592)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: イネ / トランスポゾン / 転移因子 / 変異創生 / 優性変異

Outline of Final Research Achievements

To analyze functions of unknown genes of rice, it is required to develop effective gene analysis system which carry the new mutaion. Rice endogenous DNA transposon, nDart elements were non-autonomous element which were transposed by transpoase protein expressed by an autonoumous aDart1-27. Most of insertion sites identifed of nDart were found at genic region. nDart/aDart1-27 system was induced into a rice variety Koshihikari, named as nDart-Koshi-Tag line. The rice endogenous DNA transposon nDart elements were successfuly exploited as mutant line. 3000 MK-1 plants per year were grown for three years. For reverse genetic analysis, identification of insertion sites of nDart have advanced.

Free Research Field

植物分子遺伝