Construction of efficient genetic analysis model for apple breeding by production of doubled haploids.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25450038
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Horticultural science
• Research Institution: Iwate University
• Principal Investigator: KOMORI Sadao 岩手大学, 農学部, 教授 (00333758)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: リンゴ / Malus × domestica Borkh. / 葯培養 / 倍加半数体

Outline of Final Research Achievements

All processes of “embryo formation”, “shoot induction” and “shoot multiplication and acclimatization” are important for obtaining DHs in apple anther cultures. It is important for efficient acquisition of DHs to select cultivars with high differentiation and multiplication capability in the processes. ‘Senshu’, ‘Starking Delicious’ and ‘Sekaiichi’ were selected as the cultivars suitable for anther culture. It was clarified that the sampling of flower bud at late “Tight clustre” to early “First pink” stage which mainly contained “early uninucleate” to “late uninucleate” microspore and chilling treatment for 25 to 35 days of flower buds were efficient for increasing embryo formation. The seedlings which used DH as a parent were raised, and it was proven that the simplification of mode of inheritance was possible. Acquired DHs were cultivated in the field, and the individual with fertility was found.

Free Research Field

果樹園芸学