2015 Fiscal Year Final Research Report
Functions of PEPCK and gluconeogenesis in regulation of sugar /acid balance and sugar accumulation in tomato fruit
| Project/Area Number |
25450039
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Horticultural science
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | PEPCK / トマト / 果実成分 / 糖新生 / 糖蓄積 / 実生生長 / 糖酸比 |
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| Outline of Final Research Achievements |
PEPCK is known as a key regulatory enzyme in the gluconeogenesis pathway in plants. However, its physiological function in whole plant development including fruit sugar accumulation is not fully understood in plants with fresh berry-type fruit. To elucidate this function, we generated transgenic tomato plants in which PEPCK is over-expressed or down-regulated. Detailed characterization of the transgenic lines, it was revealed that PEPCK and gluconeogenesis are involved in the regulation of sugar/acid balance in developing tomato fruit. Additionally, PEPCK expression level also affected initial seedling growth. In the PEPCK-overexpressing lines, exogenous sucrose supply strongly promoted post-germination growth. Those results indicate PEPCK is involved in not only regulation in carbohydrate supply but also its availability in tomato seedling.
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| Free Research Field |
蔬菜生理学、遺伝育種学、糖・アミノ酸代謝
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