Studies on a plant virus replication complexes as the transportation hub

2015 Fiscal Year Final Research Report

• Project/Area Number: 25450058
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant protection science
• Research Institution: Kyoto University
• Principal Investigator: Kaido Masanori 京都大学, (連合)農学研究科(研究院), 助教 (20314247)
• Co-Investigator(Renkei-kenkyūsha): TANIGUCHI Hisaaki 徳島大学, 疾患酵素学研究センター, 教授 (10257636)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: RNAウイルス / 細胞間移行 / 複製複合体 / 小胞体膜 / 宿主因子 / 移行タンパク質

Outline of Final Research Achievements

Using affinity purification and mass spectrometry, we have identified a host protein GAPDH-A from Nicotiana benthamiana plants as the interacting partner of the movement protein (MP) of Red clover necrotic mosaic virus (RCNMV). GAPDH-A colocalized with virus replication complex (VRC) of RCNMV on the endoplasmic reticulum. We revealed that GAPDH-A is the host factor protein that contributes to the viral cell-to-cell movement, through its function to recruit the MP to VRCs. Furthermore, we showed the cortical punctate structures that contain the MP and VRC are required for the viral cell-to-cell movement and are not required for the replication of viral genomic RNA.

Free Research Field

植物病理学