New development of non-isotope heavy metals as alternative electron stains of uranyl acetate for plant pathology samples

2015 Fiscal Year Final Research Report

• Project/Area Number: 25450059
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant protection science
• Research Institution: Kobe University
• Principal Investigator: Park Pyoyun 神戸大学, (連合)農学研究科(研究院), 名誉教授 (20147094)
• Co-Investigator(Kenkyū-buntansha): IKEDA Kenichi 神戸大学, 大学院・農学研究科, 准教授 (40437504) ; PARK Moon-Son 神戸大学, 大学院・農学研究科, 学術推進員 (50600417)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 酢酸ウラン / 透過電子顕微鏡 / 非放射性重金属剤 / 塩化ハフニウム / ガドリ二ウム塩 / サマリウム塩 / 細胞コントラスト / ネガティブ染色

Outline of Final Research Achievements

Uranyl salts are effective to observe the cells of sections from plant pathology samples with　much polysaccharides, but not to increase the contrast of cell walls, starch grains, glycogen particles and collagen fibers. Because of the radioactive salts, the use and purchase have been restricted only in Japan. The non-isotope heavy metals, positive to stain both the cell structures and the polysaccharides, are requested now. The staining effects of 5 different metals (SmCl3, SmAc3, GdCl3, GdAc3 and HfCl4) was examined ultrastructurally in cells of plant leaves and roots, insects, and fungi. Among them, GdAc3 and HfCl4 were excellent as positive electron stains whereas GdCl3 was good as a negative stain.

Free Research Field

農学