2015 Fiscal Year Final Research Report
Analysis of dwarf and constitutive defense responses activated by loss of specific Arabidopsis MAP kinase pathway involved in innate immunity signaling
| Project/Area Number |
25450060
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant protection science
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| Research Institution | Kagawa University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | サプレッサー変異体 / MAPキナーゼ経路 / 防御反応 / 矮性 |
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| Outline of Final Research Achievements |
Mitogen-activated protein kinase (MAPK) pathways have a pivotal role in innate immunity signaling in plants. Arabidopsis MEKK1, a MAPK kinase kinase, constitutes specific pathway with MKK1/MKK2 and MPK4. This pathway has an important role in regulation of defense gene expression in response to microbe associated molecular patterns recognition. However, loss of MEKK1 results in constitutive defense responses accompanying severe dwarfism and temperature-dependent cell death. To elucidate the complex mechanism underlying the mekk1 phenotypes, we performed genetic screening to isolate suppressor mutant of the dwarf and defense phenotypes. With the aid of the MutMap analysis, we identified the candidate of causal gene for the mutant. Genetic analysis by producing double mutant with mekk1 or mpk4, smn1 mutation suppressed the dwarf and defense phenotypes of these mutants. These results showed that SMN1 positively contributed to the phenotypes.
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| Free Research Field |
植物病理学
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