Molecular analysis and characterization of the broad-spectrum cyst nematode resistant tomato

2015 Fiscal Year Final Research Report

• Project/Area Number: 25450063
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant protection science
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: UEHARA Taketo 国立研究開発法人農業・食品産業技術総合研究機構, 中央農業研究センター虫・鳥獣害研究領域, 上級研究員 (30355458)
• Co-Investigator(Renkei-kenkyūsha): MASUTA Chikara 北海道大学, 大学院農学研究院, 教授 (60281854)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: トマト / Globodera / HeroA / タバコシストセンチュウ / 抵抗性遺伝子

Outline of Final Research Achievements

①We confirmed that Potato Cyst Nematode (PCN)-resistant tomato cultivars in Japan contained the HeroA gene, whereas susceptible cultivars did not. ②PCN-resistant tomato cultivars are identical to Tabacco Cyst Nematode (TCN)-resistant tomato cultivars. ③When segregated progenies from the selfing of resistant cultivar (Doctor-K) were inoculated with PCN, the progenies segregated into 20 resistant and five susceptible plants, fitting the 3 : 1 ratio expected for single-gene inheritance (χ2=0.61, P=0.56). ④Microarray analyses were performed to investigate the transcriptome profiles in the incompatible and compatible interactions at 3 and 7 days postinoculation (dpi).The PR1(P4,P6) transcript increased to exceptionally high level at 3 dpi in the cyst nematode-infected resistant plants compared to the uninoculated controls. ⑤We elucidated the effective of cyst nematode-resistant tomato cultivars in reducing the population of TCN in greenhouse traials.

Free Research Field

植物寄生性線虫