2015 Fiscal Year Final Research Report
Development of self-cloning of symbiotic genes of rhizobium
| Project/Area Number |
25450085
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | Kagawa University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 遺伝子解析 / 共生窒素固定 / 根粒代謝 |
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| Outline of Final Research Achievements |
We surveyed proteome and RNA seq analysis of nodule tissue formation and during aging using a legume plant (Lotus japonicum) and a rhizobium (Mesorhizobium loti). Cluster analysis of these gene expression profiles suggested the tissue and age specific metabolic genes of these organisms. The function of the tissue specific genes were surveyed using transgenic Lotus japonicum/meosrhizobium system. GS1 (glutamine synthetase I) gene of the M. loti was especially curious because the mutant showed strong effect to stumulate aging of the nodules. We studied the function of the gene and developed the technique to perform self-cloning of the gene in rhizobium.
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| Free Research Field |
植物栄養学
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