Exploration of bifidobacterial genes involved in the intestinal survival through a dynamics analysis of random transposon mutants of Bifidobacterium longum

2015 Fiscal Year Final Research Report

• Project/Area Number: 25450090
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Hokkaido University
• Principal Investigator: FUKIYA Satoru 北海道大学, (連合)農学研究科(研究院), 講師 (10370157)
• Co-Investigator(Kenkyū-buntansha): OGURA Yoshitoshi 九州大学, 大学院医学研究院, 准教授 (40363585)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: トランスポゾン変異導入 / 転移因子 / Bifidobacterium longum / INSeq法 / 腸内生存 / ビフィズス菌 / プロモーター / ゲノム解析

Outline of Final Research Achievements

Bifidobacteria, which exert health-promoting effects, are one of the beneficial members of the human intestinal microbiota. Molecular mechanisms of their intestinal survival are still unclear due to the insufficient development of gene-mutagenesis systems in this genus. This study aimed to identify bifidobacterial genes that contribute to the intestinal survival using combined analysis of a transposon mutagenesis and next-generation sequencing.
In this study, the complete genomic sequence of the mutagenesis-host strain, Bifidobacterium longum 105-A, was determined. Two different transposon mutagenesis systems were developed using transposable elements ISBlo11, derived from B. longum, and Himar1C9. This progress will pave the way to clarify the molecular mechanisms of bifidobacterial survival in the intestine.

Free Research Field

応用微生物学・腸内細菌学