Screening and identification of substretes of processing peptidases from Aspergillus oryzae

2015 Fiscal Year Final Research Report

• Project/Area Number: 25450096
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: Youhei Yamagata 東京農工大学, (連合)農学研究科(研究院), 教授 (40230338)
• Co-Investigator(Kenkyū-buntansha): TAKEUCHI Michio 東京農工大学, 大学院農学研究院, 教授 (50092490)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 麹菌 / kexin / プロセッシングプロテアーゼ / ポリリン酸キナーゼ

Outline of Final Research Achievements

We developed a new pull-down method to detect substrates for the processing protease of Japanese representative industry microbe, Aspergillus oryzae. According to the method, we detected a candidate protein. The protein was an orthologue of yeast polyphosphate kinase. The enzyme is reported concerning with responses to the oxidative stress and starvation stress and maintenance of the intracellular phosphate concentration in yeast. It had been thought deletion of A. oryzae kexin gene caused defective processing of the proteins related to the cell wall synthesis, and it gave rise to growth suppression. However, our results seemed to indicate kexin activated the important proteins involved in the homeostatic system in A. oryzae different from previously what we though.

Free Research Field

応用酵素学