2016 Fiscal Year Final Research Report
Comparative genomic analysis of intestinal bacteria involved in isoflavone metabolism
| Project/Area Number |
25450098
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Gifu University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2017-03-31
|
| Keywords | 腸内細菌 / エクオール / イソフラボン / ゲノム / 遺伝子マーカー |
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| Outline of Final Research Achievements |
Nucleotide sequences of 3 equol-producing bacteria derived from culture collection were analyzed and comparative genome analysis was performed together with 2 other known bacterial genomes and 2 gene cluster related to equol metabolism. The comparative genome analysis indicated that the genes are divided into 3 phylogenic groups. In addition, PCR primers were designed from the gene cluster and real time PCR was carried out using the bacterial genomes as templates, it was confirmed that amplification was carried out with the same efficiency. Furthermore, feces of 5 equol producers and 5 non-producers were collected and amplification was attempted using the total extracted genome as a template, using the same primers, amplification of only equol producers was confirmed.
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| Free Research Field |
微生物学
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