Improvement of Saccharomyces cerevisiae for bioethanol production by modified genome shuffling

2015 Fiscal Year Final Research Report

• Project/Area Number: 25450100
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Osaka University
• Principal Investigator: Sugiyama Minetaka 大阪大学, 工学(系)研究科(研究院), 准教授 (80379130)
• Co-Investigator(Kenkyū-buntansha): HARASHIMA Satosi 崇城大学, 生物生命学部, 教授 (70116086) ; 笹野 佑 大阪大学, 大学院工学研究科, 助教 (90640194)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: Saccharomyces cerevisiae / Thermotolerance / Bioethanol

Outline of Final Research Achievements

In this study, we tried to improve thermotolerance of S.cerevisiae by modified genome shuffling. In the first round of genome shuffling, S. cerevisiae TJ14 and C3867 strains showing thermotolerance up to 41°C with high ethanol productivity were used as recipient strains. Non-conventional yeast Hansenula polymorpha showing robust thermotolerance up to 49°C was chosen as donor strains. After transformation of recipient strains with genomic DNA from donor strain, 3 transformants were isolated at 42°C and they showed pretty better growth and 7 times higher ethanol production than their recipient strains at 42°C. In the second round of genome shuffling, 2 transformants were isolated at 42.5°C and they showed significantly better growth and 2 times higher ethanol production than their recipient strains at 42.5°C. These strains will contribute to providing better platforms for further development of thermotolerant yeast for bioethanol production.

Free Research Field

酵母分子遺伝学