Application of a phosphite dehydrogenase gene as a dominant selection marker for a large-scale cultivation of microorganisms

2015 Fiscal Year Final Research Report

• Project/Area Number: 25450102
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Hiroshima University
• Principal Investigator: Ryuichi Hirota 広島大学, 先端物質科学研究科, 助教 (90452614)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 亜リン酸 / 選択マーカー / 微生物 / 培養

Outline of Final Research Achievements

The use of antibiotic resistance markers in the commercial application of genetically modified microorganisms is limited due to restrictions on the release of antibiotics and their resistance genes to the environment. To avoid contamination by other microorganisms, the development of a dominant selection marker with low environmental risks is still required. In this study, we developed a novel selection system for microorganisms using a bacterial phosphite dehydrogenasegene (PtxD). Introduction of PtxD gene into a fission yeast Schizosaccharomyces pombe, a budding yeast Saccharomyces cerevisiae, and a Gram-negative bacterium Escherichia coli could confer the ability to grow on a minimal medium containing phosphite (Pt) as a sole source of phosphorus. Since Pt is a safe and inexpensive chemical, ptxD could be used as a novel dominant selection marker applicable to a large-scale cultivation of microorganisms.

Free Research Field

応用微生物学