Functional analysis of plant pathogen effector proteins in yeast and its application

2015 Fiscal Year Final Research Report

• Project/Area Number: 25450104
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Kagawa University
• Principal Investigator: Tabuchi Mitsuaki 香川大学, 農学部, 准教授 (00294637)
• Co-Investigator(Kenkyū-buntansha): TANAKA NAOTAKA 香川大学, 農学部, 准教授 (60324109)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 病原菌エフェクター / グルタチオン / 酵母 / チオレドキシン / 青枯病菌

Outline of Final Research Achievements

The plant pathogenic bacterium Ralstonia solanacearum injects more than 70 effector proteins into the host plant cells via needle-like structure of a type Ⅲ secretion system. The effector proteins manipulate host cellular functions with diverse molecular activities. The understanding of the molecular function of effectors is essential to uncover the molecular mechanism for pathogenesis of R. solanacearum-infection. Here, we show that a ChaC-like domain containing effector RipAY from R. solanacearum exhibits γ-glutamyl cyclotransferase (GGCT) activity to degrade the major intracellular redox buffer, glutathione, which is known to be crucial for a proper activation of host immune defense in plants. Intriguingly, RipAY protein exhibits a robust GGCT activity by the interaction with eukaryotic thioredoxins, which are also important for intracellular redox homeostasis during bacterial infection in plants.

Free Research Field

応用微生物学