Characterization of transcriptional factors involved in the unique properties of a white koji mold

2015 Fiscal Year Final Research Report

• Project/Area Number: 25450106
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Kagoshima University
• Principal Investigator: FUTAGAMI Taiki 鹿児島大学, 農水産獣医学域農学系, 准教授 (60512027)
• Co-Investigator(Kenkyū-buntansha): GOTO Masatoshi 九州大学, 大学院農学研究院, 准教授 (90274521)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 白麹菌 / Aspergillus / 転写制御因子 / クエン酸

Outline of Final Research Achievements

The transcriptional change of a white koji mold, Aspergillus luchuensis mut. kawachii, during barley koji production was investigated and 7 putative transcriptional factor encoding genes (nosA、rosA、atfA、atfB、AKAW_01957, AKAW_02766, AKAW_02903) were identified among the genes with significant transcriptional changes. These genes were disrupted and the nosA and rosA double disruptant showed radial colony growth similar to the wild type strain, but showed reduced conidia formation. It was also indicated that the NosA-GFP and RosA-GFP fusion proteins localized in the nuclei.

Free Research Field

応用微生物学