Isolation of intracellular symbiotic bacteria of chemotroph.

2016 Fiscal Year Final Research Report

• Project/Area Number: 25450122
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Japan Agency for Marine-Earth Science and Technology
• Principal Investigator: NOGI Yuichi 国立研究開発法人海洋研究開発機構, 海洋生命理工学研究開発センター, 技術副主幹 (70399559)
• Research Collaborator: KOYAMA Sumihiro 国立研究開発法人海洋研究開発機構, 海洋生物多様性分野, 主任研究員
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 共生菌 / ハオリムシ / 難培養微生物

Outline of Final Research Achievements

We have established a method for isolating intracellular or extracellular symbiotic bacteria that was difficult until now. As a result, we isolated and cultivated intracellular and extracellular symbiotic microorganisms of tubeworm (deep sea organism). Weak electric current was used to efficiently isolate intracellular symbiotic bacteria alive. Confirmation that the isolated bacteria are symbiotic intracellular symbiotic cells of tubeworm was performed with the 16S rRNA gene. Furthermore, many of the strains isolated as extracellular symbiotic bacteria were novel species. Of these, we identified new species of genus Ruegeria, Sulfitobacter and Planktotalea. The adsorptive isolation method based on the weak current used in this study was considered to be one means for culturing difficult-to-cultivate microorganisms in the future.

Free Research Field

微生物