Development of high-throughput method for the susceptibility testing of microbial biofilm and screening of biofilm eradication activity

2015 Fiscal Year Final Research Report

• Project/Area Number: 25450123
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Fukuoka Industrial Technology Center
• Principal Investigator: Tsukatani Tadayuki 福岡県工業技術センター, 生物食品研究所, 専門研究員 (50463500)
• Co-Investigator(Renkei-kenkyūsha): KAWAGUCHI Tomoaki 福岡県工業技術センター, 生物食品研究所, 主任技師 (90455337)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: バイオフィルム / 薬剤感受性試験 / 微生物検出 / スクリーニング

Outline of Final Research Achievements

High-throughput method for the susceptibility testing of microbial biofilm using a colorimetric microbial viability assay based on reduction of a water soluble tetrazolium salt and the biofilm formation method on 96 pegs on the lid of a microtiter plate was developed. By the proposed method, bifilm MIC, MBC and MEC were sequentially detemined. The proposed method yields similar performance to conventional methods, but is faster and more easily implemented. The water and ethanol extracts of various materials (mainly foodstuffs) were investigated for the biofilm eradication activity using the proposed method. Among them, the ethanol extracts of rosemary leaf, udo peel, mace, iroko, and red pine showed potent biofilm eradication activity for both S. mutans and S. aureus. Furthermore, these materials combined with poly-L-lysine, which was used as a food additive, most effectively reduced the biofilm MECs for a wide variety of bacteria.

Free Research Field

微生物検出