Substrate recognition of bacterial copper-containing amine oxidase possessing broad substrate specifictiy

2016 Fiscal Year Final Research Report

• Project/Area Number: 25450129
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Shimane University
• Principal Investigator: SAWA Yoshihiro 島根大学, 生物資源科学部, 教授 (70127489)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: アミンオキシダーゼ / バイオセンサー / 基質認識 / 分子設計

Outline of Final Research Achievements

We tried to develop biosensor enzymes useful for the specific quantification of physiologically active amines such as GABA, tyramine, histamine, by elucidating the substrate recognition mechanism of two bacterial copper-containing amine oxidases possessing broad substrate specificity.
Unfortunately we could not develop a promising enzyme as a biosensor, but in silico analysis shows a high correlation with biochemical results and it is at a stage where it can be fully utilized for the molecular design of amine oxidase.

Free Research Field

タンパク質工学