Production of useful compounds using bamboo cultured cells by rational metabolic-flow switching.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25450134
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Toyama Prefectural University
• Principal Investigator: KATO Yasuo 富山県立大学, 工学部, 教授 (20254237)
• Co-Investigator(Kenkyū-buntansha): OGITA SHINJIRO 富山県立大学, 工学部, 准教授 (50363875) ; NOMURA TAIJI 富山県立大学, 工学部, 講師 (40570924)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 植物二次代謝産物 / 生合成 / タケ培養細胞 / ヒドロキシ桂皮酸アミド / 代謝フロースイッチング

Outline of Final Research Achievements

We surveyed secondary metabolites occurring mainly in bamboo cells and identified feruloylputrescine and p-coumaroylputrescine, suggesting that phenylpropanoid and polyamine biosynthetic pathways are highly active, and the bamboo cells are suitable for the production of alternative secondary metabolites derived from those pathways. We next generated stable transformant of bamboo cells expressing agmatine coumaroyltransferase gene of barley in the expectation of metabolic-flow switching from hydroxycinnamoylputrescines to hydroxycinnamoylagmatines. As a result, in the recombinant cells, the putrescine amides content decreased and instead high content of agmatine amides was newly produced as expected, where the content of major product p-coumaroylagmatine reached approximately 350 mg per liter of culture. The results provide proof-of-concept to the usefulness of ‘rational metabolic-flow switching’ in synthetic biology using plant cell hosts.

Free Research Field

植物生化学