2015 Fiscal Year Final Research Report
Elucidation of catalytic mechanisms of thermostable glycoside-hydrolases by structural analyses
| Project/Area Number |
25450143
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
MIne Shouhei 国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (70415751)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | グルコサミン / 好熱菌 / 酵素 |
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| Outline of Final Research Achievements |
Chitin is a polysaccharide that comprises a major constituent of fungal cell walls, the exoskeletons of insects, and the shells of crustaceans. N-acetylglucosamine (GlcNAc) and Glucosamine (GlcN), which is derived from the hydrolysis of chitin or chitosan,have a variety of biological functions and have therefore been used as a food additive and in medicines. Using the chitin catabolic pathway of hyperthemophilie,we have focused two thermostable enzymes, deacetylase (Dac) (EC 3.5.1.-) and β-N-acetylglucosaminidases (NagA) (EC 3.2.1.52). To understand the catalysis and adaptation to extreme high temperature of these enzymes, we have determined the structures of Dac and NagA.
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| Free Research Field |
構造生物学
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