Design, syntheses and diagnostic applications of beta(1-6)-linked glycolipids (GGLs) as the major cell membrane components of Mycoplasma pneumoniae

2015 Fiscal Year Final Research Report

• Project/Area Number: 25450146
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bioorganic chemistry
• Research Institution: Chiba University
• Principal Investigator: NISHIDA Yoshihiro 千葉大学, 融合科学研究科(研究院), 教授 (80183896)
• Research Collaborator: MATSUDA Kazuhiro エムバイオテック株式会社 ; MATSUDA Sachie エムバイオテック株式会社 ; YUAN Mengfei 千葉大学, 大学院融合科学研究科, 博士課程 ; FUKUDA Kazuo 千葉大学, 大学院融合科学研究科, 博士課程 ; TANAKA Daiki 千葉大学, 大学院融合科学研究科, 博士課程 ; DOHI Hirofumi 千葉大学, 大学院融合科学研究科, 准教授 ; UZAWA Hirotaka 産業技術総合研究所, ナノシステム研究部門, 主任研究員 ; MIZUNO Mamoru 公益財団法人野口研究所, 糖鎖有機化学研究室, 室長
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 糖鎖 / マイコプラズマ / 感染診断 / 細胞膜 / 糖脂質 / グリコシル化反応 / 三次元構造 / 脂質抗原

Outline of Final Research Achievements

Mycoplasma is one of the simplest bacteria having no rigid cell walls and lipopolysaccharides (LPS). Instead, they utilize either alpha- or beta-glycolipids in cytoplasmic membrane. Also in our preceding studies, we have reported alpha-glycolipid antigens (GGPLs) in M. fermentans as well as beta-linked ones (GGLs)in M. pneumoniae. In the present study, we applied our glycosylation methods for the library assembly of GGLs homologues carrying variable acyl chains and examined their chemical behaviors. The chemical library involved a deuterium labeled GGL homologue for simultaneous MS detection of natural GGLs. It involved also an acetyl (C2) homologue for the study on molecular dynamics (MD). The assembled chemical library enabled us to elucidate the best GGLs homologue (GGLS C18) for the ELISA diagnosis of Mycoplasma pneumoniae infection and their possibility to make a crown ether-like ionophore around the non-reducing terminal end in their dynamic three dimensional (3D) structures.

Free Research Field

生物有機化学