Establishment of pollen formation suppression techniques of Japanese ceder using highly safe surfactant

2015 Fiscal Year Final Research Report

• Project/Area Number: 25450219
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Forest science
• Research Institution: Tokyo University of Agriculture
• Principal Investigator: Koshio Kaihei 東京農業大学, 国際食料情報学部, 教授 (50318177)
• Co-Investigator(Kenkyū-buntansha): HIRATSUKA Rie 東京慈恵会医科大学, 医学部, 准教授 (30246376)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 花粉症 / スギ / 界面活性剤 / プログラム細胞死

Outline of Final Research Achievements

New techniques to kill male flowers of Cryptomeria japonica were tried to establish by the application of sorbitan trioleate which is a food additive and used for cosmetics. By the observation of TEM, it was clear that sorbitan trioleate induced necrosis type programmed cell death of male flower cells leading to the suppression of pollen formation. The cells treated with sorbitan trioleate seemed to shrink separated from cell wall and cell membrane was broken so that cytosol was leaked out to apoplast. The electrophoresis of DNA showed smear degradation. Sorbitan trioleate induces plant growth by suppression of male flowers and cones of Cryptomeria japonica, which can lead to establish a practical technique to decrease pollen dissemination without damaging the values of Japanese ceder timbers.

Free Research Field

森林科学、植物生理