2015 Fiscal Year Final Research Report
Development of the method to identify the gene involved in fruiting body formation of Lentinula edodes
| Project/Area Number |
25450245
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Wood science
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KAMEI Ichiro 宮崎大学, 農学部, 教授 (90526526)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | シイタケ / 子実体形成 / RNAi / 形質転換 / 光受容体 / きのこ |
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| Outline of Final Research Achievements |
This study developed the transformation method with RNAi construct for knockdown of specific gene involved in the fruiting-body formation of edible mushroom Lentinula edodes. Photoreceptor gene A of L. edodes (Le.phrA) was cloned and 600 bp of coding region was used for RNAi construct ligated with the partial sequence of GUS gene. RNAi construct for Le. phrA was successfully introduced with selection marker vector into protoplast of L. edodes. Some transformants showed brown color mycelium, and that the growth was inhibited remarkably. This phenotype might be caused by RNAi knockdown of Le. phrA.
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| Free Research Field |
森林化学
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