Response of the family Vibrionaceae in the intestinal tracts of fish subjected to the stressful conditions

2015 Fiscal Year Final Research Report

• Project/Area Number: 25450263
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Aquatic bioproduction science
• Research Institution: Nihon University
• Principal Investigator: SUGITA Haruo 日本大学, 生物資源科学部, 教授 (50139052)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 腸内細菌叢 / トラフグ / プロバイオティクス / ビブリオ科細菌 / 飼育密度 / 飼育温度 / 配合飼料 / ｑＰＣＲ

Outline of Final Research Achievements

(1) As a model system of intestinal contents of pufferfish (Takifugu rubripes), the slurry which was prepared by mixing the grinded feeds and intestinal contents of pufferfish was evaluated. However, the bacterial community in the slurry was remarkably different from that of feeds and intestinal contents, suggesting that this system needs to be improved for the study of intestinal microbiology of pufferfish.
(2) Pufferfish was reared in glass aquaria under the stressful conditions of fish density and temperatures, and the family Vibrionaceae in the intestinal tract of pufferfish was monitored. As a result, the trend that the family Vibrionaceae was enhanced to some extent as the fish density increased in the aquaria. This fact suggests that Vibrionaceae may proliferate rapidly under stressful conditions such as high density of rearing fish.

Free Research Field

農学