Structure and function of MytiLec-1, an alpha-galactose-binding lectin from the mussel Mytilus galloprovincialis.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25450306
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Aquatic life science
• Research Institution: Yokohama City University
• Principal Investigator: Ozeki Yasuhiro 横浜市立大学, 生命ナノシステム科学研究科, 教授 (70275022)
• Research Collaborator: Imtiaj Hasan 横浜市立大学, 大学院, 博士後期課程 ; Koide Yasuhiro 横浜市立大学, 大学院, 博士後期課程 ; Kawsar S. M. Abe JSPS, 外国人招へい研究者 ; Fujii Yuki 長崎国際大学, 薬学部, 助教
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: レクチン / MytiLec-1 / MytiBase / トランスクリプト―ム / 海産無脊椎動物 / ムラサキイガイ / α-ガラクトース / 細胞内情報伝達

Outline of Final Research Achievements

Whole structural information including the gene, mRNA (GenBank LC125182) and 3-D structures (PDB 3wu and 3wv) of MytiLec-1, an alpha-galactose-binding lectin from the Mediterranean mussel Mytilus galloprovincialis have been provided by transcriptome, cloning and X-ray crystallographic analysis, respectively in addition to the primary structure (B3EWR1). Structural properties of MytiLec-1 have two exons in the gene, alternative-N-terminal extension in the full-length mRNA and five essential amino acids in subunit of the polypeptide. MytiLec-1 multiply activated both classic MAP-kinase (MEK/ERK) and stress activated kinase (JNK and p38) pathways in addition to the activation of caspase-9/3. The potency of MytiLec-1 suggested that the lectin has a possibility to act as a pattern recognition receptor which induce cellular regulations of the mussel cells.

Free Research Field

糖鎖生物学