2015 Fiscal Year Annual Research Report
魚類免疫機構における重要なサイトカインIL-2/15/15Lファミリーの解明
| Project/Area Number |
25450310
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| Research Institution | Fujita Health University |
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Principal Investigator |
J・M Dijkstra 藤田保健衛生大学, 総合医科学研究所, 講師 (10387681)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | fish / cytokine / interleukin / evolution / IL-2 / IL-15 / IL-15L / IL-5 |
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| Outline of Annual Research Achievements |
Achievements fiscal year 2013-2015 (1-04-2013 to 1-04-2016):
For IL-2/15/15L cytokines we found that the trout cytokines IL-2, IL-15, and IL-15L cross-react with IL-15Ra across fish-mammal borders, and vice versa, except for mammalian IL-2 which only binds mammalian IL-2Ra. We found that trout cytokines IL-2, IL-15, and IL-15L are N-glycosylated and have functional effects on trout lymphocytes, and that they can signal through chimera receptor chains on co-transfected cells which consist of rainbow trout IL-2Rb, IL21Ra or IL-2Rg ectodomains that were combined with the transmembrane + cytoplasmic domains of the corresponding bovine receptor chains. We are currently trying to repeat the effect by adding exogenous instead of co-transfected trout cytokines, and so finally determine the receptor for IL-15L. We found that recombinant carp IL-2 can help with the establishment of permanent T cell cultures. We were the first to find bona fide genes of the IL-5 cytokine family in teleost fish, and also found that many mammals have the ancient cytokine KK34 which so far was only known in chicken.
The following results were achieved in the fiscal year 2015 (1-04-2015 to 1-04-2016):
(1) Detection of the probable signaling receptors for trout cytokines including for IL-15L. (2) The proliferative effect of carp IL-2 on T cell cultures. (3) Writing of a review on fish immune milieus and cytokines. (4) The first detection of IL-5 family genes in teleost fish. (5) The detection of a hitherto unknown but ancient cytokine in mammals, KK34.
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Research Products
(3 results)
