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2015 Fiscal Year Final Research Report

Elucidation of the important IL-2/15 /15L cytokine family functions in the fish immune system

Research Project

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Project/Area Number 25450310
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Aquatic life science
Research InstitutionFujita Health University

Principal Investigator

Dijkstra Johannes Martinus  藤田保健衛生大学, 総合医科学研究所, 講師 (10387681)

Co-Investigator(Renkei-kenkyūsha) 中西 照幸  日本大学, 生物資源科学部, 教授 (00322496)
Project Period (FY) 2013-04-01 – 2016-03-31
KeywordsFish / Immunology / Evolution / Cytokine / Interleukin 2 / Interleukin 15 / Interleukin 15-like / Receptor
Outline of Final Research Achievements

For IL-2/15/15L cytokines we found that the trout cytokines IL-2, IL-15, and IL-15L cross-react with IL-15Ra across fish-mammal borders, and vice versa, except for mammalian IL-2 which only binds mammalian IL-2Ra. We found that trout cytokines IL-2, IL-15, and IL-15L are N-glycosylated and have functional effects on trout lymphocytes, and that they can signal through chimera receptor chains on co-transfected cells which consist of rainbow trout IL-2Rb, IL-21Ra or IL-2Rg ectodomains that were combined with the transmembrane + cytoplasmic domains of the corresponding bovine receptor chains. We are currently trying to repeat the effect by adding exogenous instead of co-transfected trout cytokines. In addition, we found that recombinant carp IL-2 can help with the establishment of permanent T cell cultures. Furthermore, we were the first to find bona fide genes of the IL-5 cytokine family in teleost fish.

Free Research Field

Immunology, Evolution, Fish

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Published: 2017-05-10  

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