2015 Fiscal Year Final Research Report
Elucidation of the important IL-2/15 /15L cytokine family functions in the fish immune system
| Project/Area Number |
25450310
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Aquatic life science
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| Research Institution | Fujita Health University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
中西 照幸 日本大学, 生物資源科学部, 教授 (00322496)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | Fish / Immunology / Evolution / Cytokine / Interleukin 2 / Interleukin 15 / Interleukin 15-like / Receptor |
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| Outline of Final Research Achievements |
For IL-2/15/15L cytokines we found that the trout cytokines IL-2, IL-15, and IL-15L cross-react with IL-15Ra across fish-mammal borders, and vice versa, except for mammalian IL-2 which only binds mammalian IL-2Ra. We found that trout cytokines IL-2, IL-15, and IL-15L are N-glycosylated and have functional effects on trout lymphocytes, and that they can signal through chimera receptor chains on co-transfected cells which consist of rainbow trout IL-2Rb, IL-21Ra or IL-2Rg ectodomains that were combined with the transmembrane + cytoplasmic domains of the corresponding bovine receptor chains. We are currently trying to repeat the effect by adding exogenous instead of co-transfected trout cytokines. In addition, we found that recombinant carp IL-2 can help with the establishment of permanent T cell cultures. Furthermore, we were the first to find bona fide genes of the IL-5 cytokine family in teleost fish.
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| Free Research Field |
Immunology, Evolution, Fish
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