Research and development of the light controlling technique to produce periwinkle alkaloids high-efficiently

2015 Fiscal Year Final Research Report

• Project/Area Number: 25450373
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Agricultural environmental engineering/Agricultural information engineering
• Research Institution: Tamagawa University
• Principal Investigator: KANEKO Keiko (大橋敬子) 玉川大学, 学術研究所, 教授 (50332599)
• Co-Investigator(Kenkyū-buntansha): HIRATA Kazumasa 大阪大学, 大学院薬学研究科, 教授 (30199062)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: ニチニチソウ / アルカロイド / 植物工場 / 発光ダイオード / 赤色光 / UV-A光 / 光強度 / 環境制御

Outline of Final Research Achievements

In this study, we cultivated C. roseus in an enclosed artificial lighting plant factory and tried to develop the light controlling technique to improve production of vinblastine which was widely used in cancer chemotherapy. Red light compared with blue light and a mixture of red light and blue light (red PPFD: blue PPFD = 1:1) promoted to the growth and the concentrations of the monomeric precursors of vindoline and catharanthine in leaves. Vinblastine was accumulated in the leaves of the plants irradiated with UV-A light for 5 d and its content was increased until UV-A light intensity reached 10 W/m2. Vinblastine content in the younger leaves was greater than that in the older leaves. In conclusion, we constructed cultivation methods for efficient vinblastine production, as follows. Firstly, C. roseus plants are grown under 150 micro mol/m2/s red light only for one month, and then these plants are irradiated to red light supplemented with UV-A light of 10 W/m2 for 7 d.

Free Research Field

生物環境工学、植物栄養学