Development of methods to evaluate molecular characteristics of livestock spermatozoa by the detection of aberrant fertility-regulating factors

2015 Fiscal Year Final Research Report

• Project/Area Number: 25450394
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Animal production science
• Research Institution: Kobe University
• Principal Investigator: HARAYAMA HIROSHI 神戸大学, (連合)農学研究科(研究院), 教授 (30281140)
• Co-Investigator(Kenkyū-buntansha): MURASE Tetsuma 岐阜大学, 応用生物科学部, 教授 (30303514) ; FUKUSHIMA Moriyuki 兵庫県立農林水産技術総合センター, 北部農業技術センター, 主任研究員 (60463395)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 応用動物 / 畜産学 / 雄性繁殖能力 / 精子 / 人工授精 / ウシ / ブタ

Outline of Final Research Achievements

The aim of the present study was to develop a new method to evaluate sperm molecular characteristics for identifying AI-subfertile males of livestock. In this study, we succeeded in the development of the method to evaluate molecular characteristics of bull (Japanese Black bull) cryopreserved and freshly ejaculated spermatozoa by the observation of acrosomal tyrosine-phosphorylated proteins. Moreover, we also showed that outcomes of AI and in vitro fertilization can be predicted by this evaluation for the spermatozoa.

Free Research Field

生殖生物学