2015 Fiscal Year Final Research Report
Physiological and genetical study of bird prolactin regulatory element binding protein
| Project/Area Number |
25450401
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Animal production science
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| Research Institution | Azabu University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 下垂体 / 転写因子 / プロラクチン / 繁殖周期 / 就巣 |
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| Outline of Final Research Achievements |
Expression of chicken and turkey prolactin regulatory element binding protein in anterior pituitary gland was investigated during embryogenesis and reproductive cycle. Levels of PREB mRNA generally show the similar profile to PRL mRNA. Especially, high level of PREB mRNA was detected during incubation stage. Since high level PRL mRNA was induced by hypothalamic VIP during incubation stage, effects of VIP on mRNA expression of PREB in the anterior pituitary gland of embryo and laying stage were examined. Levels of PREB and PRL mRNAs were not changed by stimulation of VIP in the anterior pituitary gland of embryonic days of 14. However, induced effects of forskolin was observed in the embryonic day 14.On the other hands, stimulatory effects of VIP and forskolin were observed in the anterior pituitary gland of embryonic day 20. This result clearly indicating that effects of VIP mainly regulated by the mRNA levels of VIP receptor in the anterior pituitary gland.
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| Free Research Field |
応用動物科学
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