Identification of IgY receptor responsible for IgY uptakes into egg yolks by comprehensive RNA sequencing analysis

2015 Fiscal Year Final Research Report

• Project/Area Number: 25450407
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Animal production science
• Research Institution: Nagoya University
• Principal Investigator: Murai Atsushi 名古屋大学, 生命農学研究科, 准教授 (10313975)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 畜産物 / 卵 / ニワトリ / ウズラ / IgY / 抗体 / 受容体 / 網羅的発現解析

Outline of Final Research Achievements

The present study was conducted to identify an IgY receptor contributing IgY uptake into avian egg yolks. Analysis of comprehensive RNA sequence in chicken ovarian follicles showed expression of 9,848 transcripts. Three already known IgY receptors were included in these transcripts, but their expression levels were low. To proceed an efficient selection of candidate gene from whole transcripts, chicken or quail groups possessing novel IgY uptake ability were explored. IgY-deficient chickens subjected to brusectomy increased IgY uptakes into egg yolks by 3-fold, suggesting usefulness of this chickens model for comprehensive RNA sequencing analysis. No remarkable changes were observed in ability of IgY uptakes into egg yolks among six quail stains.

Free Research Field

動物栄養生理学